Ontology highlight
ABSTRACT:
SUBMITTER: Marsic T
PROVIDER: S-EPMC8056947 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Marsic Tin T Ali Zahir Z Tehseen Muhammad M Mahas Ahmed A Hamdan Samir S Mahfouz Magdy M
Nano letters 20210412 8
Rapid, sensitive, and specific point-of-care testing for pathogens is crucial for disease control. Lateral flow assays (LFAs) have been employed for nucleic acid detection, but they have limited sensitivity and specificity. Here, we used a fusion of catalytically inactive SpCas9 endonuclease and VirD2 relaxase for sensitive, specific nucleic acid detection by LFA. In this assay, the target nucleic acid is amplified with biotinylated oligos. VirD2-dCas9 specifically binds the target sequence via ...[more]