De novo design of transmembrane β barrels.
Ontology highlight
ABSTRACT:
SUBMITTER: Vorobieva AA
PROVIDER: S-EPMC8064278 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
De novo design of transmembrane β barrels.
Vorobieva Anastassia A AA White Paul P Liang Binyong B Horne Jim E JE Bera Asim K AK Chow Cameron M CM Gerben Stacey S Marx Sinduja S Kang Alex A Stiving Alyssa Q AQ Harvey Sophie R SR Marx Dagan C DC Khan G Nasir GN Fleming Karen G KG Wysocki Vicki H VH Brockwell David J DJ Tamm Lukas K LK Radford Sheena E SE Baker David D
Science (New York, N.Y.) 20210201 6531
Transmembrane β-barrel proteins (TMBs) are of great interest for single-molecule analytical technologies because they can spontaneously fold and insert into membranes and form stable pores, but the range of pore properties that can be achieved by repurposing natural TMBs is limited. We leverage the power of de novo computational design coupled with a "hypothesis, design, and test" approach to determine TMB design principles, notably, the importance of negative design to slow β-sheet assembly. We ...[more]
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