Dataset Information

The Effects of Drought and Re-Watering on Non-Structural Carbohydrates of Pinus tabulaeformis Seedlings.

ABSTRACT: Intense and frequent drought events strongly affect plant survival. Non-structural carbohydrates (NSCs) are important "buffers" to maintain plant functions under drought conditions. We conducted a drought manipulation experiment using three-year-old Pinus tabulaeformis Carr. seedlings. The seedlings were first treated under different drought intensities (i.e., no irrigation, severe, and moderate) for 50 days, and then they were re-watered for 25 days to explore the dynamics of NSCs in the leaves, twigs, stems, and roots. The results showed that the no irrigation and severe drought treatments significantly reduced photosynthetic rate by 93.9% and 32.6% for 30 days, respectively, leading to the depletion of the starch storage for hydraulic repair, osmotic adjustment, and plant metabolism. The seedlings under moderate drought condition also exhibited starch storage consumption in leaves and twigs. After re-watering, the reduced photosynthetic rate recovered to the control level within five days in the severe drought group but showed no sign of recovery in the no irrigation group. The seedlings under the severe and moderate drought conditions tended to invest newly fixed C to starch storage and hydraulic repair instead of growth due to the "drought legacy effect". Our findings suggest the depletion and recovery of starch storage are important strategies for P. tabulaeformis seedlings, and they may play key roles in plant resistance and resilience under environmental stress.

SUBMITTER: Guo X

PROVIDER: S-EPMC8066268 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Non-structural carbohydrates (NSCs) facilitate plant adaptation to drought stress, characterize tree growth and survival ability, and buffer against external disturbances. Previous studies have focused on the distribution and dynamics of NSCs among different plant organs under drought conditions. However, discussion about the NSC levels of fine roots in different root branch orders is limited, especially the relationship between fine root trait variation and NSC content. The objective of the study was to shed light on the synergistic variation in fine root traits and NSC content in different root branch orders under different drought and soil substrate conditions. The 2-year-old Fraxinus mandshurica Rupr. potted seedlings were planted in three different soil substrates (humus, loam, and sandy-loam soil) and subjected to four drought intensities (CK, mild drought, moderate drought, and severe drought) for 2 months. With increasing drought intensity, the biomass of fine roots decreased significantly. Under the same drought intensity, seedlings in sandy-loam soil had higher root biomass, and the coefficient of variation of 5th-order roots (37.4, 44.5, and 53% in humus, loam, and sandy-loam soil, respectively) was higher than that of lower-order roots. All branch order roots of seedlings in humus soil had the largest specific root length (SRL) and specific root surface area (SRA), in addition to the lowest diameter. With increasing drought intensity, the SRL and average diameter (AD) of all root branch orders increased and decreased, respectively. The fine roots in humus soil had a higher soluble sugar (SS) content and lower starch (ST) content compared to the loam and sandy-loam soil. Additionally, the SS and ST contents of fine roots showed decreasing and increasing tendencies with increasing drought intensities, respectively. SS and ST explained the highest degree of the total variation in fine root traits, which were 32 and 32.1%, respectively. With increasing root order, the explanation of the variation in root traits by ST decreased (only 6.8% for 5th-order roots). The observed response in terms of morphological traits of different fine root branch orders of F. mandshurica seedlings to resource fluctuations ensures the maintenance of a low cost-benefit ratio in the root system development.

Project description:Tonoplast Intrinsic Proteins (TIP) are plant aquaporins that are primarily localized in the tonoplast and play a role in the bidirectional flux of water and other substrates across a membrane. In barley, eleven members of the HvTIP gene subfamily have been identified. Here, we describe the transcription profile of the HvTIP genes in the leaves of barley seedlings being grown under optimal moisture conditions, drought stress and a re-watering phase. The applied drought stress caused a 55% decrease in the relative water content (RWC) in seedlings, while re-watering increased the RWC to 90% of the control. Our analysis showed that all HvTIP genes, except HvTIP3;2, HvTIP4;3 and HvTIP5.1, were expressed in leaves of ten-day-old barley seedlings under optimal water conditions with the transcripts of HvTIP2;3, HvTIP1;2 and HvTIP1;1 being the most abundant. We showed, for the first time in barley, a significant variation in the transcriptional activity between the analysed genes under drought stress. After drought treatment, five HvTIP genes, which are engaged in water transport, were down-regulated to varying degrees, while two, HvTIP3;1 and HvTIP4;1, were up-regulated. The HvTIP3;1 isoform, which is postulated as transporting hydrogen peroxide, expressed the highest increase of activity (ca. 5000x) under drought stress, thus indicating its importance in the response to this stress. Re-hydration caused the return of the expression of many genes to the level that was observed under optimal moisture conditions or, at least, a change in this direction Additionally, we examined the promotor regions of HvTIP and detected the presence of the cis-regulatory elements that are connected with the hormone and stress responses in all of the genes. Overall, our results suggest that 7 of 11 studied HvTIP (HvTIP1;1, HvTIP1;2, HvTIP2;1, HvTIP2;2, HvTIP2;3, HvTIP3;1, HvTIP4;1) have an important function during the adaptation of barley to drought stress conditions. We discuss the identified drought-responsive HvTIP in terms of their function in the adaptation of barley to this stress.

Dataset Information

The Effects of Drought and Re-Watering on Non-Structural Carbohydrates of Pinus tabulaeformis Seedlings.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets