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Ameliorated Antibacterial and Antioxidant Properties by Trichoderma harzianum Mediated Green Synthesis of Silver Nanoparticles.

ABSTRACT: Biosynthesis of silver nanoparticles using beneficial Trichoderma harzianum is a simple, eco-friendly and cost-effective route. Secondary metabolites secreted by T. harzianum act as capping and reducing agents that can offer constancy and can contribute to biological activity. The present study aimed to synthesize silver nanoparticles using T. harzianum cell filtrate and investigate different bioactive metabolites based on LC-MS/MS analysis. The synthesized silver nanoparticles (AgNPs) from T. harzianum were characterized by ultraviolet-visible spectrophotometry, Fourier transform infrared spectrometry (FT-IR), energy-dispersive spectroscopy (EDS), dynamic light scattering (DLS), X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The surface plasmon resonance of synthesized particles formed a peak centered near 438 nm. The DLS study determined the average size of AgNPs to be 21.49 nm. The average size of AgNPs was measured to be 72 nm by SEM. The cubic crystal structure from XRD analysis confirmed the synthesized particles as silver nanoparticles. The AgNPs exhibited remarkable antioxidant properties, as determined by DPPH and ferric reducing antioxidant power (FRAP) assay. The AgNPs also exhibited broad-spectrum antibacterial activity against two Gram-positive bacteria (S. aureus and B. subtilis) and two Gram-negative bacteria (E. coli and R. solanacearum). The minimum inhibitory concentration (MIC) of AgNPs towards bacterial growth was evaluated. The antibacterial activity of AgNPs was further confirmed by fluorescence microscopy and SEM analysis.

SUBMITTER: Konappa N

PROVIDER: S-EPMC8066458 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The flower of safflower is widely used in Chinese herbal preparations and the non-medicinal parts have been applied to develop a sustainable green method, where AgNPs were generated using a mixture of leaf and stem after 12 h of incubation in the dark. In this study, we intend to improve the efficiency of the reduction reaction and optimize this green method by selecting other non-medicinal parts, such as the bud and the pure stem, evaluating the biosynthesis parameters and harnessing the assistance of ultrasonication. Visual observation and UV-vis spectroscopy confirmed that both safflower stem (SS) and bud (SB) mediated AgNPs (SS-AgNPs and SB-AgNPs, respectively) could be produced rapidly over time under ultrasonication. An alkaline solution could accelerate the formation of SS-AgNPs and SB-AgNPs with greater surface loads. SS-AgNPs and SB-AgNPs of small size could be obtained at pH 8.0 and 10.0, respectively. Large concentrations of SS and SB extract are also beneficial for forming AgNPs of small size. It is in acid and neutral solutions that monodispersed SS-AgNPs and SB-AgNPs can be generated. Characterization of selectively synthesized SS-AgNPs and SB-AgNPs demonstrated their spherical shape with the actual size below 30 nm covered by anions. Both SS-AgNPs and SB-AgNPs exhibited potent antioxidant and antibacterial activity. The MIC values of SS-AgNPs for S. aureus and E. coli were 12.5 and 25.0 μg mL-1, respectively, slightly superior to SB-AgNPs. In an in vitro anticancer assay, both kinds of AgNPs show potent toxicity action against the SW620 cell line with IC50 values of 5.4 and 10.6 μg mL-1, respectively. However, only SS-AgNPs reveal an inhibitory action against the HeLa cell line, where the IC50 is found to be 26.8 μg mL-1. These results provide experimental proof that the assistance of ultrasonication and adjusting the process parameters are efficient methods for promoting the reduction reaction, and both SS and SB mediated AgNPs could serve as a promising antioxidant, antibacterial and anticancer agents.

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Ameliorated Antibacterial and Antioxidant Properties by Trichoderma harzianum Mediated Green Synthesis of Silver Nanoparticles.

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