Ontology highlight
ABSTRACT:
SUBMITTER: Amalina ZN
PROVIDER: S-EPMC8070779 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Amalina Zulkiply Nor ZN Khalid Muhammad Fazli MF Rahman Sjafri Faizul SF Ahmad Muhamad Nuramin MN Ahmad Najib Mohamad M Ismail Asma A Aziah Ismail I
Diagnostics (Basel, Switzerland) 20210414 4
A multiplex rapid detection system, based on a PCR-lateral flow biosensor (mPCR-LFB) was developed to identify <i>Salmonella</i> Typhi and <i>Salmonella</i> Paratyphi A from suspected carriers. The lower detection limit for <i>S</i>. Typhi and <i>S</i>. Paratyphi A was 0.16 and 0.08 ng DNA equivalent to 10 and 10<sup>2</sup> CFU/mL, respectively. Lateral flow biosensor was used for visual detection of mPCR amplicons (stgA, SPAint, ompC, internal amplification control) by labeling forward primers ...[more]