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Analysis of patchclamp recordings: model-free multiscale methods and software.

ABSTRACT: Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other.

SUBMITTER: Pein F

PROVIDER: S-EPMC8071803 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Analysis of patchclamp recordings: model-free multiscale methods and software.

Pein Florian F Eltzner Benjamin B Munk Axel A

European biophysics journal : EBJ 20210301 2

Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to ...[more]

PMID: 33837454

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