Highly efficient multiplex genetic engineering of porcine primary fetal fibroblasts.

ABSTRACT:

Background

Genetically engineered porcine donors are a potential solution for the shortage of human organs for transplantation. Incompatibilities between humans and porcine donors are largely due to carbohydrate xenoantigens on the surface of porcine cells, provoking an immune response which leads to xenograft rejection.

Materials and methods

Multiplex genetic knockout of GGTA1, β4GalNT2, and CMAH is predicted to increase the rate of xenograft survival, as described previously for GGTA1. In this study, the clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated protein 9 system was used to target genes relevant to xenotransplantation, and a method for highly efficient editing of multiple genes in primary

SUBMITTER: Klapholz B 

PROVIDER: S-EPMC8074785 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature