Dataset Information

Antihormone treatment differentially regulates PSA secretion, PSMA expression and ⁶⁸Ga-PSMA uptake in LNCaP cells.

ABSTRACT:

Background

In recent years, a variety of innovative therapeutics for castration-resistant prostate cancer have been developed, including novel anti-androgenic drugs, such as abiraterone or VPC-13566. Therapeutic monitoring of these pharmaceuticals is performed either by measuring PSA levels in serum or by imaging. PET using PSMA ligands labeled with Fluor-18 or Gallium-68 is the most sensitive and specific imaging modality for detection of metastases in advanced prostate cancer. To date, it remains unclear how PSMA expression is modulated by anti-hormonal treatment and how it correlates with PSA secretion.

Methods

We analyzed modulation of PSMA-mRNA and protein expression, ⁶⁸Ga-PSMA uptake and regulation of PSA secretion by abiraterone or VPC-13566 in LNCaP cells in vitro.

Results

We found that abiraterone and VPC-13566 upregulate PSMA protein and mRNA expression but block PSA secretion in LNCaP cells. Both anti-androgens also enhanced ⁶⁸Ga-PSMA uptake normalized by the number of cells, whereas abiraterone and VPC-13566 reduced ⁶⁸Ga-PSMA uptake in total LNCaP monolayers treated due to cell death.

Conclusion

Our data indicate that PSA secretion and PSMA expression are differentially regulated upon anti-androgen treatment. This finding might be important for the interpretation of ⁶⁸Ga-PSMA PET images in monitoring therapies with abiraterone and VPC-13566 in prostate cancer patients, but needs to be validated in vivo.

SUBMITTER: Mathy CS

PROVIDER: S-EPMC8076114 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Publications

Antihormone treatment differentially regulates PSA secretion, PSMA expression and <sup>68</sup>Ga-PSMA uptake in LNCaP cells.

Mathy C S CS Mayr T T Kürpig S S Meisenheimer M M Dolscheid-Pommerich R C RC Stoffel-Wagner B B Kristiansen G G Essler M M Muders M H MH Bundschuh R A RA

Journal of cancer research and clinical oncology 20210324 6

<h4>Background</h4>In recent years, a variety of innovative therapeutics for castration-resistant prostate cancer have been developed, including novel anti-androgenic drugs, such as abiraterone or VPC-13566. Therapeutic monitoring of these pharmaceuticals is performed either by measuring PSA levels in serum or by imaging. PET using PSMA ligands labeled with Fluor-18 or Gallium-68 is the most sensitive and specific imaging modality for detection of metastases in advanced prostate cancer. To date, ...[more]

PMID: 33760944

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Dataset Information

Antihormone treatment differentially regulates PSA secretion, PSMA expression and ⁶⁸Ga-PSMA uptake in LNCaP cells.

Background

Methods

Results

Conclusion

Publications

Antihormone treatment differentially regulates PSA secretion, PSMA expression and <sup>68</sup>Ga-PSMA uptake in LNCaP cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Antihormone treatment differentially regulates PSA secretion, PSMA expression and 68Ga-PSMA uptake in LNCaP cells.

Background

Methods

Results

Conclusion

Publications

Antihormone treatment differentially regulates PSA secretion, PSMA expression and <sup>68</sup>Ga-PSMA uptake in LNCaP cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Antihormone treatment differentially regulates PSA secretion, PSMA expression and ⁶⁸Ga-PSMA uptake in LNCaP cells.