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Rational Engineering of the DNA Walker Amplification Strategy by Using a Au@Ti₃C₂@PEI-Ru(dcbpy)₃²⁺ Nanocomposite Biosensor for Detection of the SARS-CoV-2 RdRp Gene.

ABSTRACT: The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@Ti₃C₂@PEI-Ru(dcbpy)₃²⁺ nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA-Ag nanoclusters (model DNA-AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to 100 pM and a limit of detection of 0.21 fM. It was indicated that the ECL biosensor had a great application potential for clinical medical detection. Furthermore, the DNA walker amplification also played a reliable candidate strategy for other detection methods.

SUBMITTER: Yao B

PROVIDER: S-EPMC8084271 | biostudies-literature |

REPOSITORIES: biostudies-literature

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