Unknown

Dataset Information

0

Voltage-Sensing Domain of the Third Repeat of Human Skeletal Muscle NaV1.4 Channel As a New Target for Spider Gating Modifier Toxins.


ABSTRACT: Voltage-gated sodium channels (NaV) have a modular architecture and contain five membrane domains. The central pore domain is responsible for ion conduction and contains a selectivity filter, while the four peripheral voltage-sensing domains (VSD-I/IV) are responsible for activation and rapid inactivation of the channel. "Gating modifier" toxins from arthropod venoms interact with VSDs, influencing the activation and/or inactivation of the channel, and may serve as prototypes of new drugs for the treatment of various channelopathies and pain syndromes. The toxin-binding sites located on VSD-I, II and IV of mammalian NaV channels have been previously described. In this work, using the example of the Hm-3 toxin from the crab spider Heriaeus melloteei, we showed the presence of a toxin-binding site on VSD-III of the human skeletal muscle NaV1.4 channel. A developed cell-free protein synthesis system provided milligram quantities of isolated (separated from the channel) VSD-III and its 15N-labeled analogue. The interactions between VSD-III and Hm-3 were studied by NMR spectroscopy in the membrane-like environment of DPC/LDAO (1 : 1) micelles. Hm-3 has a relatively high affinity to VSD-III (dissociation constant of the complex Kd ~6 μM), comparable to the affinity to VSD‑I and exceeding the affinity to VSD-II. Within the complex, the positively charged Lys25 and Lys28 residues of the toxin probably interact with the S1-S2 extracellular loop of VSD-III. The Hm-3 molecule also contacts the lipid bilayer surrounding the channel.

SUBMITTER: Myshkin MY 

PROVIDER: S-EPMC8084291 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5039624 | biostudies-literature
| S-EPMC5995520 | biostudies-literature
| S-EPMC5577582 | biostudies-literature
| S-EPMC3511134 | biostudies-literature
| S-EPMC2847040 | biostudies-literature
| S-EPMC2881780 | biostudies-literature
| S-EPMC8002187 | biostudies-literature
| S-EPMC2932606 | biostudies-literature
| S-EPMC4116111 | biostudies-literature
| S-EPMC9231009 | biostudies-literature