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High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells.

ABSTRACT: Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for large-scale production of uniform midbrain organoids that can be maintained in diverse formats, and our reporters allow for sorting of dopaminergic neurons. The maturing long-term organoid cultures can be used as a model for the entire midbrain. For complete details on the use and execution of this protocol, please refer to Ahfeldt et al. (2020).

SUBMITTER: Sarrafha L

PROVIDER: S-EPMC8086141 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells.

Sarrafha Lily L Parfitt Gustavo M GM Reyes Ricardo R Goldman Camille C Coccia Elena E Kareva Tatyana T Ahfeldt Tim T

STAR protocols 20210419 2

Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for large-scale production of uniform midbrain organoids that can be maintained in diverse formats, and our reporters allow for sorting of dopaminergic neurons. The maturing long-term organoid cultures can be ...[more]

PMID: 33997803

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