Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system.

ABSTRACT: We report the first combination of droplet digital and rapid PCR techniques for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. The presented rapid digital PCR system simultaneously detects two specific targets (ORF1ab and N genes) and one reference gene (RNase P) with a single PCR thermal cycling period around 7 s and the total running time less than 5 min. A clear positive signal could be identified within 115 s via the rapid digital RT-PCR, suggesting its efficiency for the end-point detection. In addition, benchmark tests with serial diluted reference samples of SARS-CoV-2 RNA reveal the excellent accuracy of our system (R²>0.99). More importantly, the rapid digital PCR system gives consistent and accurate detection of low-concentration reference samples,

SUBMITTER: Yin H 

PROVIDER: S-EPMC8093165 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature