The transcription regulator and c-di-GMP phosphodiesterase PdeL represses motility in Escherichia coli.

ABSTRACT: PdeL is a transcription regulator and catalytically active c-di-GMP phosphodiesterases (PDE) in Escherichia coli PdeL has been shown to be a transcription autoregulator, while no other target genes have been identified so far. Here, we show that PdeL represses transcription of the flagella class II operon, fliFGHIJK, and activates sslE encoding an extracellular anchored metalloprotease, among additional loci. DNA-binding studies and expression analyses using plasmidic reporters suggest that regulation of the fliF and sslE promoters by PdeL is direct. Transcription repression of the fliFGHIJK operon, encoding protein required for assembly of the flagellar basal body, results in inhibition of motility on soft agar plates and reduction of flagella ass

SUBMITTER: Yilmaz C 

PROVIDER: S-EPMC8095459 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature