Theileria, Hepatozoon and Taenia infection in great gerbils (Rhombomys opimus) in northwestern China.
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ABSTRACT:
SUBMITTER: Ji N
PROVIDER: S-EPMC8099453 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:Rhombomys opimus Genome sequencing and assembly
| PRJNA491814 | ENA
Project description:Great gerbil (Rhombomys opimus Lichtenstein, 1823) is distributed in Central Asia and some parts of the Middle East. It is widely found in central and northeast parts of Iran with two distinct genetic lineages: R. o. sodalis in the northern slopes of the Elburz Mountains and R. o. sargadensis in the southern slopes. This large rodent acts as the main host of natural focal diseases. No study has surveyed the ecological niche of the lineages and how their distribution might be influenced by different climatic variables. To examine the distribution patterns of this murid rodent, we aimed to determine the habitat preferences and effects of environmental variables on the ecological niche. Using a species distribution approach for modeling of regional niche specialization, suitable habitats predicted for R. o. sodalis were mainly located in Golestan province in northern Iran, along the northern slope of Elburz, while R. o. sargadensis, showed great potential distribution along the southern slope of Elburz and around the Kavir Desert and the Lut Desert. Despite the widest potential distribution of R. o. sargadensis from northeast to northwest and through Central Iran, the geographic range of R. o. sodalis was smaller and mostly confined to Golestan province. The results support the presence of the two genetic lineages of Rhombomys in Iran and confirm that there is no significant niche overlap between the two subspecies. Furthermore, it provided several perspectives for future taxonomic studies and prevention hygiene programs for public health.
| S-EPMC8412373 | biostudies-literature
Project description:Karimabad virus (KARV) is an arthropod-borne viral agent originally found in the Mediterranean region that can cause human infection via sandfly as the main vector. The KARV virion has been only detected from sandfly in western Asian countries and specific antibody has been detected from Rhombomys opimus and human in countries in Africa, Western and Central Asia. In this study, by next-generation sequencing (NGS) on a high variety of wild small animals in Xinjiang Autonomous Region in China, we obtained a complete sequence of KARV from Rhombomys opimus. An expanded epidemiological investigation was subsequently performed on 1713 small wild mammals that were widely collected from seven bioclimatic distinct sites in China by applying KARV specific RT-PCR and sequencing. Positive results were only obtained from 8 (2.29%) of the Rhombomys opimus captured in Xinjiang Autonomous Region, while not in 57 rodent species that were captured in other six provinces. Sequence analysis revealed the currently identified KARV was clustered with Gabek Forest virus, and they shared 79.1-93.9% identity with Iranian KARV that differed for L, M and S segments. Phylogenetic analysis based on eight partial L gene sequences demonstrated the separation of two lineages of the current KARV sequences. The first report of KARV in Rhombomys opimus in China expanded the currently known geographic scope, reservoirs types and the genetic heterogeneity of KARV. Our results show a new host, Rhombomys opimus, for KARV and highlight potential zoonotic transmission of KARV in humans.
| S-EPMC9582553 | biostudies-literature
Project description:A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil (Rhombomys opimus) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial 12S rRNA, cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. BLAST analysis of DNA sequencing indicated 99.00% homology in 12S rRNA and cox1 genes and 98.00% homology in nad1 gene with Hydatigera krepkogorski (accession No. AB731762). The sequences of current isolate were deposited in GenBank by accession Nos. MF281971, MF281972 and MF281973 for 12 SrRNA, cox1 and nad1 genes, respectively. This study was the first report of molecular characterization of H. krepkogorski from Iran. Isolation and characterization of the adult stage from definitive host will help to better clarify incomplete life cycle and morphology data of this species in the world.
| S-EPMC6346488 | biostudies-literature
Project description:BackgroundGreat gerbils, Rhombomys opimus, are the main reservoir host of zoonootic cutaneous leishmaniasis (ZCL) in Iran and neighboring countries. Based on morphological traits two subspecies R. opimus sodalis and R. opimus sargadensis have reported in the country. However, variation in infection rate and signs to Leishmania parasites, phenotype, size, and sexual polymorphisms demand more details to elucidate clearly the role of great gerbils in ZCL epidemiology.MethodsPCR-RFLP and PCR-direct sequencing were used to analyze mitochondrial DNA cytochrome B (mtDNA-cytB) gene structure of R. opimus collected from Golestan and Khorasan-e-Razavi Provinces in 2011 that are neighbor to Turkmenistan Country where ZCL is endemic in both sides of the borderline.ResultsAll of the specimens (n= 61) were morphologically or genetically similar to the typical R. opimus sodalis. However, there were 9 (1.5%) DNA substitutions throughout the 583 bp of the Cyt b gene of the samples sequenced comprising six DNA haplotypes. Maximum likelihood or neighbor joining phylogenetic trees inferred from the sequences could resolve the populations according to their subspecies as well as geographical origins.DiscussionThe DNA polymorphisms in the great gerbils may correspond to the signs and infection rate in the animal. However, further studies are needed to match these six haplotypes with different signs and parasite sustaining following infection with L. major in the great gerbils.
| S-EPMC3875884 | biostudies-literature
Project description:The Eurasian lynx (Lynx) is a medium-sized wild cat species distributed throughout Eurasia. There has been no report on Taenia species (Cestoda: Cyclophyllidea) infecting this felid in China. In this study, 24 tapeworms were found in two Eurasian lynxes (#1 and #2) in Xinjiang Uygur Autonomous Region (XUAR), northwestern China. Based on the number, measurements and the shape of rostellar hooks, these tapeworms belong to two Taenia species. According to the number (n = 32) and length (185-194 μm) of small hooks, the first Taenia species (n = 1, found in #2 lynx) was identified as Taenia laticollis. Phylogenetically, this species was clustered with T. laticollis genotype C (JX860623) based on its cytochrome c oxidase subunit 1 (cox1) and 16S rDNA sequences. The second Taenia species (n = 23, provisionally named as "Taenia sp.") may represent a potentially novel tapeworm species, because of its obvious differences in the shape and lengths (174-182 μm, 98-113 μm) of large and small rostellar hooks in comparison with ten taxonomically related species. Molecular and phylogenetic analyses of the cox1 gene revealed that "Taenia sp." has the highest rate of sequence identity (92.93%, 368/396 bp) with Taenia hydatigena reported from sheep (Ovis aries) in Slovakia. To sum up, a potentially novel tapeworm species, "Taenia sp.", is found in Eurasian lynx. In addition, T. laticollis was found for the first time in China.
| S-EPMC8505354 | biostudies-literature
Project description:In this study, 263 tapeworms were collected from eight road-killed red foxes in Xinjiang Uygur Autonomous Region (XUAR, northwestern China). The tapeworms were analyzed based on morphological characters and mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences. Eighty-three Taenia and 180 Mesocestoides tapeworms were identified according to the presence or absence of rostellum, and the number, the length and the shape of the large rostellar hooks. The morphological and molecular analyses revealed that i) 180 Mesocestoides tapeworms, here named as Mesocestoides sp. (Vulpes vulpes), showed 99.21% (378/381 bp) identity to Mesocestoides sp. reported from red fox in Mongolia; and ii) 83 Taenia tapeworms belonged to three species. The first Taenia species [n = 16, named as Taenia sp. (Vulpes vulpes)], based on the length of large rostellar hooks (337–342 μm) and its cox1 sequence, was identified as a potentially novel species, which is phylogenetically close to Taenia laticollis. The second species [n = 54, named as Taenia sp. (Vulpes vulpes & Rhombomys opimus)], was morphologically similar to Taenia endothoracicus according to the number (n = 52), the length (319–332 μm) and the shape of the large rostellar hooks. This species, infecting three red foxes, shared 100% cox1 sequence identity with Taenia sp. (Rhombomys opimus) genotype C found previously in great gerbils (Rhombomys opimus) in the same region. The third species (n = 13, named as Taenia polyacantha-like), had shorter large rostellar hooks (178–180 μm) and showed 96.27% (361/375 bp) sequence identity to Taenia polyacantha reported from red fox in Italy. The “great gerbil-red fox” life cycle of Taenia sp. (Vulpes vulpes & Rhombomys opimus), belonging to the mitochondrial lineage of T. endothoracicus, is confirmed. The T. polyacantha-like species was firstly found in red fox in China. Taenia sp. (Vulpes vulpes) is a potentially novel species, which is close to T. laticollis based on its phylogenetic properties. Graphical abstract Image 1 Highlights • The life cycle of Taenia sp. (Vulpes vulpes & Rhombomys opimus) is confirmed.• The Taenia polyacantha-like species was firstly found in China.• Taenia sp. (Vulpes vulpes) is a potentially novel species in China.
| S-EPMC8626562 | biostudies-literature
Project description:BACKGROUND: Theileria and Babesia protozoan parasites are transmitted mainly by tick vectors. These parasites cause heavy economic losses to the live-stock industry, as well as affecting the health of wild animals in parasite-endemic areas. Identification of infectious agents in wild animals is not only crucial for species preservation, but also provides valuable information on parasite epidemiology. Here, we conducted a molecular surveillance study in Northwestern China to assess the prevalence of blood pathogens in cervids. METHODS: PCR analysis and microscopic evaluation of blood smears to detect Theileria- and Babesia-related diseases in Cervidae were conducted, in which 22 blood samples from red deer (n?=?22) in Qilian Mountain and 20 from sika deer (n?=?20) in Long Mountain were collected and tested for the presence of Theileria and Babesia. The 18S rRNA gene was amplified, and selected polymerase chain reaction (PCR)-positive samples were sequenced for species identification. RESULTS: PCR revealed that 9.1% of the Qilian Mountain samples and 20% of the Long Mountain samples were positive for Theileria uilenbergi; 90.09% of the Qilian Mountain samples (n?=?22) were positive for T. capreoli, but all of the Long Mountain samples (n?=?20) were negative for T. capreoli; no other Theileria or Babesia species were found. PCR showed that T. uilenbergi and T. capreoli were present in red deer in Qilian Mountain, while only T. uilenbergi was found in sika Deer in Long Mountain. The 18S rRNA gene sequences were aligned against the corresponding GenBank sequences of known isolates of Theileria and Babesia and subjected to phylogenetic analysis. The phylogenetic tree showed that the newly isolated Theileria spp. could be classified as belonging to two clades: one group belonged to the same clade as T. uilenbergi, the other to a clade containing T. capreoli. CONCLUSIONS: Our results provide important data to increase understanding of the epidemiology of Cervidae theileriosis, and will assist with the implementation of measures to control theileriosis transmission to Cervidae and small ruminants in central China.
| S-EPMC4029935 | biostudies-literature
Project description:We report here a human case of Taenia asiatica infection which was confirmed by genetic analyses in Dali, China. A patient was found to have symptoms of taeniasis with discharge of tapeworm proglottids. By sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene, we observed nucleotide sequence identity of 99% with T. asiatica and 96% with T. saginata. Using the cytochrome b (cytb) gene, 99% identity with T. asiatica and 96% identity with T. saginata were found. Our findings suggest that taeniasis of people in Dali, China may be mainly caused by T. asiatica.
| S-EPMC4792321 | biostudies-literature
Project description:BackgroundTheileria spp. are tick-borne protozoan parasites of the Phylum Apicomplexa, Order Piroplasmida that infect a wide range of wild and domestic animals. In Great Britain, Theileria spp. have been reported from livestock associated with transmission by the tick Haemaphysalis punctata. However, these reports have not been associated with disease. This study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north Kent.FindingsA polymerase chain reaction-reverse line blot assay indicated the presence of Theileria spp. in blood samples from five animals. Subsequent testing with a pan-piroplasm PCR of a larger panel of blood samples detected a piroplasm amplicon in 19 of 21 sheep submitted from the affected flock. Automated sequencing confirmed that these amplicons shared 99-100% identity with T. luwenshuni.ConclusionsThe clinical and PCR data suggest infection with T. luwenshuni was associated with disease and mortality in this flock.
| S-EPMC4831081 | biostudies-literature