Overexpression of TNFα induces senescence, autophagy and mitochondrial dysfunctions in melanoma cells.

ABSTRACT:

Background

Tumor necrosis factor alpha (TNFα) is a pleiotropic cytokine with both anti-tumorigenic and pro-tumorigenic activity, affecting tumor cell biology, the balance between cell survival and death. The final effect of TNFα is dependent on the type of malignant cells, with the potential to arrest cancer progression.

Methods

In order to explain the diverse cellular response to TNFα, we engineered melanoma and colorectal carcinoma cell lines stably overexpressing this cytokine.

Results

Under the TNFα overexpression, significant upregulation of two genes was observed: proinflammatory cytokine IL6 gene in melanoma cells A375 and gene for pro-apoptotic ligand TRAIL in colorectal carcinoma cells HT29, both mediated by TNFα/TNFR1 signaling. Malignant melanoma line A375

SUBMITTER: Tyciakova S 

PROVIDER: S-EPMC8101174 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature