Factorial design-assisted reversed phase-high performance liquid chromatography method for simultaneous determination of fluconazole, itraconazole and terbinafine.

ABSTRACT: A 2³ full factorial design model was used for the development of a new high performance liquid chromatography method with UV detection to estimate three antifungal drugs simultaneously. Fluconazole (FLU), itraconazole (ITR) and terbinafine (TRH) are co-administered for severe fungal infections. They have been determined using MOS-1 Hypersil C₁₈ column and an isocratic eluent; methanol 95% and phosphate buffer 5% with 0.001% triethylamine. The pH was adjusted to 7, and the flow rate was 0.7 ml min^-1. The three drugs were separated within less than 7 min at 210 nm. The developed method gave a linear response over 5-80 µg ml^-1, 5-50 µg ml^-1 and 1-50 µg ml^-1 for FLU, ITR and TRH, respectively. It showed detection limits of 0.88,

SUBMITTER: Roshdy A 

PROVIDER: S-EPMC8103232 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature