Maternal obesity is associated with phenotypic alterations in fetal immune cells by single-cell mass cytometry.

ABSTRACT:

Problem

Prenatal exposure to metabolic dysregulation arising from maternal obesity can have negative health consequences in post-natal life. To date, the specific effects of maternal obesity on fetal immunity at a cellular level have not been well characterized.

Method of study

Using cord blood mononuclear cells (CBMCs) and cord plasma (n = 9/group) isolated from infants born to women with a high body mass index (BMI>25kg/m² ) compared to women with a normal BMI (18-25kg/m² ), we evaluated differences in immune cell populations using single-cell mass cytometry (CyTOF). CBMCs were matched according to potentially confounding variables, such as maternal and gestational age, ethnicity, smoking status, and gravidity. Statistical results were adjusted for fe

SUBMITTER: Enninga EAL 

PROVIDER: S-EPMC8109213 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature