Manual and Automated Syntheses of the N-Linked Glycoprotein Core Glycans.

ABSTRACT: Presented herein are two complementary approaches to the synthesis of the core N-glycan pentasaccharide. The first, a traditional manual approach in solution, makes use of the H-bond-mediated aglycone delivery method for the highly diastereoselective introduction of the β-mannosidic linkage at room temperature. The synthesis of the core pentasaccharide was also accomplished using an high-performance liquid chromatography-assisted automated approach. The overall assembly was swift (8 h) and efficient (31%).

SUBMITTER: Pistorio SG 

PROVIDER: S-EPMC8114088 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature