Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains.

ABSTRACT: Deinococcus metallilatus MA1002 was exposed to ultraviolet radiation to generate mutants with enhanced biofilm production. Two strains (nos 5 and 6) were then selected based on their high biofilm formation, as well as their possession of higher concentrations of extracellular matrix components (eDNA, protein and saccharides) than the wild-type (WT). Genomic sequencing revealed the presence of large genome deletions in a secondary chromosome in the mutants. Expression analyses of the WT and mutant strains indicated the upregulation of genes associated with exopolysaccharide synthesis and stress response. The mutant strains showed high mortality in glucose-supplemented (TYG) medium; however, cell death and biofilm formation were not increased in mutant cells grown under acetate- or gl

SUBMITTER: Park C 

PROVIDER: S-EPMC8116681 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature