Vitamin D₃ suppresses intestinal epithelial stemness via ER stress induction in intestinal organoids.

ABSTRACT:

Background

Vitamin D₃ is important for normal function of the intestinal epithelial cells (IECs). In this study, we aimed to investigate the effects of vitamin D₃ on the differentiation, stemness, and viability of healthy IECs in intestinal organoids.

Methods

Intestinal organoids derived from mouse small intestine were treated with vitamin D₃, and the effects on intestinal stemness and differentiation were evaluated using real-time PCR and immunofluorescence staining of the distinct lineage markers. Cell viability was analyzed using viability and apoptosis assays.

Results

Vitamin D₃ enhanced IEC differentiation into the distinct lineages of specialized IECs, including Paneth, goblet, and enteroendocrine cells and absorptive enterocytes. Decreased expression levels of leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) and the presence of several LGR5-green fluorescent protein (GFP)-positive cells were observed in vitamin D₃-treated organoids derived from LGR5-GFP mice. The formation of the crypt-villus structure was also inhibited by vitamin D₃, suggesting that vitamin D₃ suppresses intestinal cell stemness. Furthermore, the expression levels of unfolded protein response genes, C/EBP homologous protein (CHOP), and activating transcription factor 6 (ATF6) were upregulated in vitamin D₃-treated organoids. Moreover, vitamin D₃ promoted apoptotic cell death in intestinal cells, which may be associated with the decrease in intestinal stemness. LGR5 gene expression, ISC number, and apoptotic cell death were partially recovered in the presence of the ER stress inhibitor tauroursodeoxycholic acid (TUDCA), suggesting that intestinal stemness suppression and intestinal apoptosis occurred via ER stress activation.

Conclusions

Our study provides important insights into the effects of vitamin D₃ on the induction of IEC differentiation and apoptotic cell death, and inhibition of intestinal stemness accompanied by ER stress augmentation.

SUBMITTER: Sittipo P 

PROVIDER: S-EPMC8117327 | biostudies-literature |

REPOSITORIES: biostudies-literature