Ontology highlight
ABSTRACT:
SUBMITTER: Umekawa M
PROVIDER: S-EPMC8119236 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Umekawa Midori M Hamada Kaito K Isono Naoto N Karita Shuichi S
Journal of applied glycoscience 20201120 4
Hexokinases catalyze glucose phosphorylation at the first step in glycolysis in eukaryotes. In the budding yeast <i>Saccharomyces cerevisiae</i> , three enzymes for glucose phosphorylation have long been known: Hxk1, Hxk2, and Glk1. In this study, we focus on Emi2, a previously uncharacterized hexokinase-like protein of <i>S. cerevisiae</i> . Our data show that the recombinant Emi2 protein (rEmi2), expressed in <i>Escherichia coli</i> , possesses glucose-phosphorylating activity in the presence ...[more]