Characterization of a Novel Caveolin Modulator That Reduces Vascular Permeability and Ocular Inflammation.

ABSTRACT:

Purpose

Caveolin (Cav) regulates various aspect of endothelial cell signaling and cell-permeable peptides (CPPs) fused to domains of Cav can reduce retinal damage and inflammation in vivo. Thus, the goal of the present study was to identify a novel CPP that improves delivery of a truncated Cav modulator in vitro and in vivo.

Methods

Phage display technology was used to identify a small peptide (RRPPR) that was internalized into endothelial cells. Fusions of Cav with the peptide were compared to existing molecules in three distinct assays, vascular endothelial growth factor-A (VEGF) induced nitric oxide (NO) release, VEGF induced vascular leakage, and in a model of immune mediated uveitis.

Results

RRPPR was internalized efficiently and was potent in blocking NO release

SUBMITTER: Bernatchez PN 

PROVIDER: S-EPMC8132009 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature