Unknown

Dataset Information

0

Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.


ABSTRACT: Natural antibodies (Abs) can target host glycans on the surface of pathogens. We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen. 2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization. Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques. FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike. FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans. Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.

SUBMITTER: Williams WB 

PROVIDER: S-EPMC8135257 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7554730 | biostudies-literature
| S-EPMC4278586 | biostudies-literature
| S-EPMC5491220 | biostudies-literature
| S-EPMC4358778 | biostudies-literature
2023-07-01 | GSE207247 | GEO
| S-EPMC3875178 | biostudies-literature
| S-EPMC6411795 | biostudies-literature
| S-EPMC8203731 | biostudies-literature
| S-EPMC7664941 | biostudies-literature
| S-EPMC5526850 | biostudies-other