Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing.
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ABSTRACT:
SUBMITTER: Krishnarjuna B
PROVIDER: S-EPMC8136615 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
Publications
Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing.
Krishnarjuna Bankala B Yamazaki Toshio T Anantharamaiah G M GM Ramamoorthy Ayyalusamy A
Chemical communications (Cambridge, England) 20210416 39
Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation. ...[more]
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