Fully Automated Sample Processing and Analysis Workflow for Low-Input Proteome Profiling.

ABSTRACT: Recent advances in sample preparation and analysis have enabled direct profiling of protein expression in single mammalian cells and other trace samples. Several techniques to prepare and analyze low-input samples employ custom fluidics for nanoliter sample processing and manual sample injection onto a specialized separation column. While being effective, these highly specialized systems require significant expertise to fabricate and operate, which has greatly limited implementation in most proteomic laboratories. Here, we report a fully automated platform termed autoPOTS (automated preparation in one pot for trace samples) that uses only commercially available instrumentation for sample processing and analysis. An unmodified, low-cost commercial robotic pipetting platform was utilized for

SUBMITTER: Liang Y 

PROVIDER: S-EPMC8140400 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature