Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms.

ABSTRACT: MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for S. cerevisiae and other fungi which takes only three hours. Modified protocols were developed for wild fungi and mammalian cells. This method for rapidly producing sequencing-ready nucleosome footprints from several organisms makes MNase-seq faster and easier, with less chemical waste.

SUBMITTER: McKnight LE 

PROVIDER: S-EPMC8141940 | biostudies-literature |

REPOSITORIES: biostudies-literature