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Draft Genome Sequence of Bacillus velezensis BZR 277, a Prospective Biocontrol Agent against Phytoparasitic Nematodes.

ABSTRACT: Bacillus velezensis strain BZR 277 is a rhizobacterium isolated from the rhizoplane of a winter oilseed rape plant from the Krasnodar region in Russia. This study presents the genome sequence of the rhizobacterium Bacillus velezensis BZR 277, which exhibited high antagonistic activity against the root-knot nematode Meloidogyne incognita Kof. The data on the genome sequence can help to identify pathways for the biosynthesis of metabolites responsible for nematicidal activity in order to improve crop protection against plant-parasitic nematodes.

SUBMITTER: Asaturova AM

PROVIDER: S-EPMC8142575 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:IntroductionSouthern blight, caused by Sclerotium rolfsii, poses a serious threat to the cultivation of Coptis chinensis, a plant with significant medicinal value. The overreliance on fungicides for controlling this pathogen has led to environmental concerns and resistance issues. There is an urgent need for alternative, sustainable disease management strategies.MethodsIn this study, Bacillus velezensis LT1 was isolated from the rhizosphere soil of diseased C. chinensis plants. Its biocontrol efficacy against S. rolfsii LC1 was evaluated through a confrontation assay. The antimicrobial lipopeptides in the fermentation liquid of B. velezensis LT1 were identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS). The effects of B. velezensis LT1 on the mycelial morphology of S. rolfsii LC1 were examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).ResultsThe confrontation assay indicated that B. velezensis LT1 significantly inhibited the growth of S. rolfsii LC1, with an inhibition efficiency of 78.41%. MALDI-TOF-MS analysis detected the presence of bacillomycin, surfactin, iturin, and fengycin in the fermentation liquid, all known for their antifungal properties. SEM and TEM observations revealed that the mycelial and cellular structures of S. rolfsii LC1 were markedly distorted when exposed to B. velezensis LT1.DiscussionThe findings demonstrate that B. velezensis LT1 has considerable potential as a biocontrol agent against S. rolfsii LC1. The identified lipopeptides likely contribute to the antifungal activity, and the morphological damage to S. rolfsii LC1 suggests a mechanism of action. This study underscores the importance of exploring microbial biocontrol agents as a sustainable alternative to chemical fungicides in the management of plant diseases. Further research into the genetic and functional aspects of B. velezensis LT1 could provide deeper insights into its biocontrol mechanisms and facilitate its application in agriculture.

Dataset Information

Draft Genome Sequence of Bacillus velezensis BZR 277, a Prospective Biocontrol Agent against Phytoparasitic Nematodes.

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