An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution.

ABSTRACT: We propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opposed to in a single direction, without adding further actuation components. The vacuum-driven force allows the device to avoid the formation of air bubbles and exhibit high tolerance in the surface condition. The device was then used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading and sample mixing. In this study, a series of ten-fold serial dilutions ranging from 100 to 10-4 with about 8 μL in each chamber was achieved, while the serial di

SUBMITTER: Wang A 

PROVIDER: S-EPMC8145514 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature