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Statistical optimization of experimental parameters for extracellular synthesis of zinc oxide nanoparticles by a novel haloalaliphilic Alkalibacillus sp.W7.

ABSTRACT: Green synthesis of zinc oxide nanoparticles (ZnO NPs) through simple, rapid, eco-friendly and an economical method with a new haloalkaliphilic bacterial strain (Alkalibacillus sp. W7) was investigated. Response surface methodology (RSM) based on Box-Behnken design (BP) was used to optimize the process parameters (ZnSO₄.7H₂O concentration, temperature, and pH) affecting the size of Alkalibacillus-ZnO NPs (Alk-ZnO NPs). The synthesized nanoparticles were characterized using UV-visible spectrum, X-ray diffraction (XRD), Scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX), Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and Zeta potential. The UV-Vis spectrum of ZnO NPs revealed a characteristic surface plasmon resonance (SPR) peak at 310 nm. XRD pattern confirmed the hexagonal wurtzite structure of highly pure with a crystallite size 19.5 nm. TEM proved the quasi-spherical shape nanoparticles of size ranging from 1 to 30 nm. SEM-EDX showed spherical shaped and displayed a maximum elemental distribution of zinc and oxygen. FTIR provided an evidence that the biofunctional groups of metabolites in Alkalibacillus sp.W7 supernatant acted as viable reducing, capping and stabilizing agents.

SUBMITTER: Al-Kordy HMH

PROVIDER: S-EPMC8149680 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:PurposeThe study aimed to find an effective method for fungal-mediated synthesis of zinc oxide nanoparticles using endophytic fungal extracts and to evaluate the efficiency of synthesized ZnO NPs as antimicrobial and anticancerous agents.MethodsZinc oxide nanoparticles (ZnO NPs) were produced from zinc nitrate hexahydrate with fungal filtrate by the combustion method. The spectroscopy and microscopy techniques, such as ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), and transmission electron microscopy (TEM) with selected area electron diffraction (SAED), were used to characterize the obtained product. Antibacterial activity on Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) samples was tested by broth microplate dilution technique. ZnO NPs antifungal activity was determined against plant pathogenic and regular contaminating fungi using the food-poison method. The anticancerous assay of the synthesized ZnO NPs was also investigated by cell uptake, MTT assay, and apoptosis assay.ResultsThe fungal synthesized ZnO NPs were pure, mainly hexagonal in shape and size range of 34-55 nm. The biosynthesized ZnO NPs could proficiently inhibit both Gram-positive and Gram-negative bacteria. ZnO NPs synthesized from fungal extract exhibited antifungal activity in a dose-dependent manner with a high percentage of mycelial inhibition. The cell uptake analysis of ZnO NPs suggests that a significant amount of ZnO NPs (1 μg/mL) was internalized without disturbing cancer cells' morphology. As a result, the synthesized ZnO NPs showed significant anticancer activity against cancer cells at 1 μg/mL concentration.ConclusionThis fungus-mediated synthesis of ZnO NPs is a simple, eco-friendly, and non-toxic method. Our results show that the synthesized ZnO NPs are an excellent novel antimicrobial and anticancer agent. Further studies are required to understand the mechanism of the antimicrobial, anticancerous action of ZnO NPs and their possible genotoxicity.

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Statistical optimization of experimental parameters for extracellular synthesis of zinc oxide nanoparticles by a novel haloalaliphilic Alkalibacillus sp.W7.

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