Dataset Information

Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on α-synuclein amyloid formation.

ABSTRACT: The formation of amyloid fibrils is a characterizing feature of a range of protein misfolding diseases, including Parkinson's disease. The propensity of native proteins to form such amyloid fibril, both in vitro and in vivo, is highly sensitive to the surrounding environment, which can alter the aggregation kinetics and fibrillization mechanisms. Here, we investigate systematically the influence of several representative environmental stimuli on α-synuclein aggregation, including hydrodynamic mixing, the presence of an air-water interface and sedimentation. Our results show that hydrodynamic mixing and interfacial effects are critical in promoting several microscopic steps of α-synuclein aggregation and amyloid fibril formation. The presence of an air-water interface under agitation significantly promoted primary nucleation. Secondary processes were facilitated by hydrodynamic mixing, produced by 3D rotation and shaking either in the presence or in the absence of an air-water interface. Effects of sedimentation, as investigated in a microgravity incubator, of α-synuclein lead only to minor changes on the aggregation kinetics rates in comparison to static conditions. These results forward the understanding of α-synuclein fibrillization, paving the way for the development of high-throughput assays for the screening of pharmacological approaches targeting Parkinson's disease.

SUBMITTER: Zhou J

PROVIDER: S-EPMC8152616 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on α-synuclein amyloid formation.

Zhou Jiangtao J Ruggeri Francesco S FS Zimmermann Manuela R MR Meisl Georg G Longo Giovanni G Sekatskii Sergey K SK Knowles Tuomas P J TPJ Knowles Tuomas P J TPJ Dietler Giovanni G

Chemical science 20200310 14

The formation of amyloid fibrils is a characterizing feature of a range of protein misfolding diseases, including Parkinson's disease. The propensity of native proteins to form such amyloid fibril, both <i>in vitro</i> and <i>in vivo</i>, is highly sensitive to the surrounding environment, which can alter the aggregation kinetics and fibrillization mechanisms. Here, we investigate systematically the influence of several representative environmental stimuli on α-synuclein aggregation, including h ...[more]

PMID: 34094057

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Project description:Due to climate change, Microcystis blooms occur at increasing frequencies in aquatic ecosystems worldwide. Wind-generated turbulence is a crucial environmental stressor that can vertically disperse the Microcystis surface scum, reducing its light availability. Yet, the interactions of Microcystis scum with the wind-generated hydrodynamic processes, particularly those at the air-water interface, remain poorly understood. Here, we explore the response of Microcystis (including colony size and migration dynamics) to varying magnitudes and durations of intermittent wind disturbances in a mesocosm system. The flow velocities, size of Microcystis colonies, and the areal coverage of the water surface by scum were measured through video observations. Our results demonstrate that low wind speeds increase colony size by providing a stable condition where Microcystis forms a scum layer and aggregates into large colonies at the air-water interface. In contrast, wind disturbances disperse scum and generate turbulence, resulting in smaller colonies with higher magnitudes of wind disturbance. We observed that surface scum can form rapidly following a long period (6 h) of high-magnitude (4.5 m s-1) wind disturbance. Furthermore, our results indicate reduced water surface tension caused by the presence of Microcystis, which can decrease surface flow velocity and counteract wind-driven mixing. The reduced surface tension may also drive lateral convection at the water surface. These findings suggest that Microcystis reduces surface tension, likely by releasing surface-active materials, as an adaptive response to various wind conditions. This could result in an increased rate of surface scum re-formation under wind conditions and potentially facilitate the lateral expansion of scum patches during weak wind periods. This study reveals new insights into how Microcystis copes with different wind conditions and highlights the importance of the air-water interface for Microcystis scum dynamics.

Dataset Information

Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on α-synuclein amyloid formation.

Publications

Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on α-synuclein amyloid formation.

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