Unknown

Dataset Information

0

Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse.


ABSTRACT: The formation of an immune synapse (IS) enables B cells to capture membrane-tethered antigens, where cortical actin cytoskeleton remodeling regulates cell spreading and depletion of F-actin at the centrosome promotes the recruitment of lysosomes to facilitate antigen extraction. How B cells regulate both pools of actin, remains poorly understood. We report here that decreased F-actin at the centrosome and IS relies on the distribution of the proteasome, regulated by Ecm29. Silencing Ecm29 decreases the proteasome pool associated to the centrosome of B cells and shifts its accumulation to the cell cortex and IS. Accordingly, Ecm29-silenced B cells display increased F-actin at the centrosome, impaired centrosome and lysosome repositioning to the IS and defective antigen extraction and presentation. Ecm29-silenced B cells, which accumulate higher levels of proteasome at the cell cortex, display decreased actin retrograde flow in lamellipodia and enhanced spreading responses. Our findings support a model where B the asymmetric distribution of the proteasome, mediated by Ecm29, coordinates actin dynamics at the centrosome and the IS, promoting lysosome recruitment and cell spreading.

SUBMITTER: Ibanez-Vega J 

PROVIDER: S-EPMC8155528 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6401660 | biostudies-literature
| S-EPMC2435682 | biostudies-literature
| S-EPMC5625060 | biostudies-literature
2024-04-19 | PXD044028 | Pride
| S-EPMC3351164 | biostudies-literature
| S-EPMC5642675 | biostudies-literature
| S-EPMC4291629 | biostudies-other
| S-EPMC9255268 | biostudies-literature
| S-EPMC4311132 | biostudies-literature
| S-EPMC7118148 | biostudies-literature