Regulation of Fructose 1,6-Bisphosphatase in Procyclic Form Trypanosoma brucei.

ABSTRACT: Glycolysis is well described in Trypanosoma brucei, while the importance of gluconeogenesis and one of the key enzymes in that pathway, fructose 1,6-bisphosphatase, is less understood. Using a sensitive and specific assay for FBPase, we demonstrate that FBPase activity in insect stage, procyclic form (PF), parasite changes with parasite cell line, extracellular glucose levels, and cell density. FBPase activity in log phase PF 2913 cells was highest in high glucose conditions, where gluconeogenesis is expected to be inactive, and was undetectable in low glucose, where gluconeogenesis is predicted to be active. This unexpected relationship between FBPase activity and extracellular glucose levels suggests that FBPase may not be exclusively involved in gluconeogenesis and may play an additiona

SUBMITTER: Wilkinson C 

PROVIDER: S-EPMC8157246 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature