Rapid 3-dimensional shape determination of globular proteins by mobility capillary electrophoresis and native mass spectrometry.

ABSTRACT: Established high-throughput proteomics methods provide limited information on the stereostructures of proteins. Traditional technologies for protein structure determination typically require laborious steps and cannot be performed in a high-throughput fashion. Here, we report a new medium throughput method by combining mobility capillary electrophoresis (MCE) and native mass spectrometry (MS) for the 3-dimensional (3D) shape determination of globular proteins in the liquid phase, which provides both the geometric structure and molecular mass information of proteins. A theory was established to correlate the ion hydrodynamic radius and charge state distribution in the native mass spectrum with protein geometrical parameters, through which a low-resolution structure (shape) of the protein co

SUBMITTER: Wu H 

PROVIDER: S-EPMC8159243 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature