Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract.

ABSTRACT: Six strictly anaerobic Gram-negative bacteria representing three novel species were isolated from the female reproductive tract. The proposed type strains for each species were designated UPII 199-6^T, KA00182^T and BV3C16-1^T. Phylogenetic analyses based on 16S rRNA gene sequencing indicated that the bacterial isolates were members of the genus Megasphaera. UPII 199-6^T and KA00182^T had 16S rRNA gene sequence identities of 99.9 % with 16S rRNA clone sequences previously amplified from the human vagina designated as Megasphaera type 1 and Megasphaera type 2, members of the human vaginal microbiota associated with bacterial vaginosis, preterm birth and HIV acquisition. UPII 199-6^T exhibited sequence identities ranging from 92.9 to 93.6 % with validly named Megasphaera isolates and KA00182^T had 16S rRNA gene sequence identities ranging from 92.6-94.2 %. BV3C16-1^T was most closely related to Megasphaera cerevisiae with a 16S rRNA gene sequence identity of 95.4 %. Cells were coccoid or diplococcoid, non-motile and did not form spores. Genital tract isolates metabolized organic acids but were asaccharolytic. The isolates also metabolized amino acids. The DNA G+C content for the genome sequences of UPII 199-6^T, KA00182^T and BV3C16-1^T were 46.4, 38.9 and 49.8 mol%, respectively. Digital DNA-DNA hybridization and average nucleotide identity between the genital tract isolates and other validly named Megasphaera species suggest that each isolate type represents a new species. The major fatty acid methyl esters include the following: C_12 : 0, C_16 : 0, C_16 : 0 dimethyl acetal (DMA) and summed feature 5 (C_15 : 0 DMA and/or C_14 : 0 3-OH) in UPII 199-6^T; C_16 : 0 and C_16 : 1 cis 9 in KA00182^T; C_12 : 0; C_14 : 0 3-OH; and summed feature 5 in BV3C16-1^T. The isolates produced butyrate, isobutyrate, and isovalerate but there were specific differences including production of formate and propionate. Together, these data indicate that UPII 199-6^T, KA00182^T and BV3C16-1^T represent novel species within the genus Megasphaera. We propose the following names: Megasphaera lornae sp. nov. for UPII 199-6^T representing the type strain of this species (=DSM 111201^T=ATCC TSD-205^T), Megasphaera hutchinsoni sp. nov. for KA00182^T representing the type strain of this species (=DSM 111202^T=ATCC TSD-206^T) and Megasphaera vaginalis sp. nov. for BV3C16-1^T representing the type strain of this species (=DSM 111203^T=ATCC TSD-207^T).

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC8161547 | biostudies-literature |

REPOSITORIES: biostudies-literature