Unknown

Dataset Information

0

An integrated electrocatalytic nESI-MS platform for quantification of fatty acid isomers directly from untreated biofluids.


ABSTRACT: Positional isomers of alkenes are frequently transparent to the mass spectrometer and it is difficult to provide convincing data to support their presence. This work focuses on the development of a new reactive nano-electrospray ionization (nESI) platform that utilizes non-inert metal electrodes (e.g., Ir and Ru) for rapid detection of fatty acids by mass spectrometry (MS), with concomitant localization of the C[double bond, length as m-dash]C bond to differentiate fatty acid isomers. During the electrospray process, the electrical energy (direct current voltage) is harnessed for in situ oxide formation on the electrode surface via electro-oxidation. The as-formed surface oxides are found to facilitate in situ epoxide formation at the C[double bond, length as m-dash]C bond position and the products are analyzed by MS in real-time. This phenomenon has been applied to analyze isomers of unsaturated fatty acids from complex serum samples, without pre-treatment.

SUBMITTER: Chintalapudi K 

PROVIDER: S-EPMC8162127 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7050201 | biostudies-literature
| S-EPMC7178424 | biostudies-literature
| S-EPMC6441329 | biostudies-literature
| S-EPMC5739065 | biostudies-literature
| S-EPMC8146994 | biostudies-literature
| S-EPMC4631652 | biostudies-literature
| S-EPMC9745812 | biostudies-literature
| S-EPMC4929498 | biostudies-literature
| S-EPMC4720167 | biostudies-literature
| S-EPMC6439090 | biostudies-other