Unknown

Dataset Information

0

The sporothriolides. A new biosynthetic family of fungal secondary metabolites.


ABSTRACT: The biosynthetic gene cluster of the antifungal metabolite sporothriolide 1 was identified from three producing ascomycetes: Hypomontagnella monticulosa MUCL 54604, H. spongiphila CLL 205 and H. submonticulosa DAOMC 242471. A transformation protocol was established, and genes encoding a fatty acid synthase subunit and a citrate synthase were simultaneously knocked out which led to loss of sporothriolide and sporochartine production. In vitro reactions showed that the sporochartines are derived from non-enzymatic Diels-Alder cycloaddition of 1 and trienylfuranol A 7 during the fermentation and extraction process. Heterologous expression of the spo genes in Aspergillus oryzae then led to the production of intermediates and shunts and delineation of a new fungal biosynthetic pathway originating in fatty acid biosynthesis. Finally, a hydrolase was revealed by in vitro studies likely contributing towards self-resistance of the producer organism.

SUBMITTER: Tian DS 

PROVIDER: S-EPMC8162735 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5504103 | biostudies-literature
| S-EPMC8403587 | biostudies-literature
| S-EPMC5479939 | biostudies-other
2010-02-02 | E-GEOD-19930 | biostudies-arrayexpress
2010-02-03 | GSE19930 | GEO
| S-EPMC5459663 | biostudies-literature
| S-EPMC6497799 | biostudies-literature
| S-EPMC5270730 | biostudies-literature
| S-EPMC3623715 | biostudies-literature
| S-EPMC6271215 | biostudies-literature