Dataset Information

Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models.

ABSTRACT:

Background

Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis.

Objective

Evaluate plasma amino acid concentrations in the euglycemic-hyperinsulinemic clamp (EHC) and prolonged glucose infusion (PGI) laminitis models.

Animals

Sixteen Standardbred horses.

Methods

Prospective experimental study. Plasma amino acid concentrations were measured in samples collected every 6 hours from horses that underwent a 48-hour EHC (n = 8) or 66-hour PGI (n = 8) after a 24- or 6-hour baseline period in EHC and PGI groups, respectively.

Results

Fifteen of the 20 measured amino acid concentrations decreased over time in both EHC and PGI horses (P < 0.001). The median percentage change from baseline for these amino acids was: histidine (EHC: 41.5%; PGI: 43.9%), glutamine (EHC: 51.8%; PGI: 35.3%), arginine (EHC: 51.4%; PGI: 41%), glutamic acid (EHC: 52.4%; PGI: 31.7%), threonine (EHC: 62.8%; PGI: 25.2%), alanine (EHC: 48.9%; PGI: 19.5%), proline (EHC: 56.2%; PGI: 30.3%), cystine (EHC: 34.9%; PGI: 31.2%), lysine (EHC: 46.4%; PGI: 27.8%), tyrosine (EHC: 27.5%; PGI: 16.9%), methionine (EHC: 69.3%; PGI: 50.8%), valine (EHC: 50.8%; PGI: 34.4%), isoleucine (EHC: 60.8%; PGI: 38.7%), leucine (EHC: 48.2%; PGI: 36.6%), and phenylalanine (EHC: 16.6%; PGI: 12.1%).

Conclusions and clinical importance

Hypoaminoacidemia develops in EHC and PGI laminitis models. The role of hypoaminoacidemia in the development of hyperinsulinemia-associated laminitis warrants further investigation.

SUBMITTER: Stokes SM

PROVIDER: S-EPMC8163125 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Publications

Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models.

Stokes Simon M SM Stefanovski Darko D Bertin François-René FR Medina-Torres Carlos E CE Belknap James K JK van Eps Andrew W AW

Journal of veterinary internal medicine 20210311 3

<h4>Background</h4>Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis.<h4>Objective</h4>Evaluate plasma amino acid concentrations in the euglycemic-hyperinsulinemic clamp (EHC) and prolonged glucose infusion (PGI) laminitis mod ...[more]

PMID: 33704816

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Dataset Information

Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models.

Background

Objective

Animals

Methods

Results

Conclusions and clinical importance

Publications

Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models.

Similar Datasets

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