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Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.

ABSTRACT: Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

SUBMITTER: Frawley AT

PROVIDER: S-EPMC8163400 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.

Frawley Andrew T AT Wycisk Virginia V Xiong Yaoyao Y Galiani Silvia S Sezgin Erdinc E Urbančič Iztok I Vargas Jentzsch Andreas A Leslie Kathryn G KG Eggeling Christian C Anderson Harry L HL

Chemical science 20200810 33

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal mi ...[more]

PMID: 34123149

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