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Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues.

ABSTRACT: Here, we describe a protocol to construct, calibrate, and operate a versatile tiling light sheet microscope for imaging cleared tissues. The microscope uses adjustable tiling light sheets to achieve higher spatial resolution and better optical sectioning ability than conventional light sheet microscopes and to image cleared tissues with the cellular to the subcellular spatial resolution. It is compatible with all tissue clearing methods and aligned semiautomatically through the phase modulation of the illumination light. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020).

SUBMITTER: Feng R

PROVIDER: S-EPMC8164092 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues.

Feng Ruili R Wang Dongyue D Chen Yanlu Y Lu Jing J Gao Liang L

STAR protocols 20210519 2

Here, we describe a protocol to construct, calibrate, and operate a versatile tiling light sheet microscope for imaging cleared tissues. The microscope uses adjustable tiling light sheets to achieve higher spatial resolution and better optical sectioning ability than conventional light sheet microscopes and to image cleared tissues with the cellular to the subcellular spatial resolution. It is compatible with all tissue clearing methods and aligned semiautomatically through the phase modulation ...[more]

PMID: 34095860

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