Ontology highlight
ABSTRACT:
SUBMITTER: Dhandapani G
PROVIDER: S-EPMC8175343 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Dhandapani Gunasekaran G Wachtel Ellen E Das Ishita I Sheves Mordechai M Patchornik Guy G
Scientific reports 20210603 1
The research described in this report seeks to present proof-of-concept for a novel and robust platform for purification of antibody fragments and to define and optimize the controlling parameters. Purification of antigen-binding F(ab')<sub>2</sub> fragments is achieved in the absence of chromatographic media or specific ligands, rather by using clusters of non-ionic detergent (e.g. Tween-60, Brij-O20) micelles chelated via Fe<sup>2+</sup> ions and the hydrophobic chelator, bathophenanthroline ( ...[more]