Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing.

ABSTRACT: Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing. For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021).

SUBMITTER: Lee S 

PROVIDER: S-EPMC8176364 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature