Dataset Information

ITRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings.

ABSTRACT:

Background

As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper.

Methods

To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress.

Result

In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

SUBMITTER: Wang J

PROVIDER: S-EPMC8180192 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings.

Wang Jing J Liang Chengliang C Yang Sha S Song Jingshuang J Li Xuefeng X Dai Xiongze X Wang Fei F Juntawong Niran N Tan Fangjun F Zhang Xilu X Jiao Chunhai C Zou Xuexiao X Chen Wenchao W

PeerJ 20210603

<h4>Background</h4>As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper.<h4>Methods</h4>To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress.<h4>Result ...[more]

PMID: 34141478

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