CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18.

ABSTRACT: Dunaliella salina (D. salina) has been exploited as a novel expression system for the field of genetic engineering. However, owing to the low or inconsistent expression of target proteins, it has been greatly restricted to practical production of recombinant proteins. Since the accurate gene editing function of clustered regularly interspaced short palindromic repeat (CRISPR)/Cas system, β-carotene hydroxylase gene was chosen as an example to explore D. salina application with the purpose of improving expression level of foreign genes. In this paper, based on pKSE401 backbone, three CRISPR/Cas9 binary vectors were constructed to targeting exon 1 and 3 of the β-carotene hydroxylase of D. salina CCAP19/18 (Dschyb). D. salina mutants were obtained by salt gradient transformation method, and t

SUBMITTER: Hu L 

PROVIDER: S-EPMC8185118 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature