Unknown

Dataset Information

0

Generating CAR T cells from tumor-infiltrating lymphocytes.


ABSTRACT: Background: Tumor-infiltrating lymphocytes (TILs) and chimeric antigen receptor (CAR) T-cell therapies have demonstrated promising, though limited, efficacy against melanoma. Methods: We designed a model system to explore the efficacy of dual specific T cells derived from melanoma patient TILs by transduction with a Her2-specific CAR. Results: Metastatic melanoma cells in our biobank constitutively expressed Her2 antigen. CAR-TIL produced greater amounts of IFN compared with parental TIL, when co-cultured with Her2 expressing tumor lines, including autologous melanoma tumor lines, although no consistent increase in cytotoxicity by TIL was afforded by expression of a CAR. Results of an in vivo study in NSG mice demonstrated tumor shrinkage when CAR-TILs were used in an adoptive cell therapy protocol. Conclusion: Potential limitations of transduced TIL in our study included limited proliferative potential and a terminally differentiated phenotype, which would need addressing in further work before consideration of clinical translation.

SUBMITTER: Mills JK 

PROVIDER: S-EPMC8186112 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3462483 | biostudies-literature
| S-DIXA-D-1003 | biostudies-other
| S-EPMC4622931 | biostudies-literature
| S-EPMC4707343 | biostudies-literature
| S-EPMC10760159 | biostudies-literature
| S-EPMC3278789 | biostudies-other
| S-EPMC4026017 | biostudies-other
| S-EPMC8319109 | biostudies-literature
2023-02-20 | PXD036811 | Pride
| S-EPMC5353900 | biostudies-literature