Spatial distribution of lamin A/C determines nuclear stiffness and stress-mediated deformation.

ABSTRACT: While diverse cellular components have been identified as mechanotransduction elements, the deformation of the nucleus itself is a critical mechanosensory mechanism, implying that nuclear stiffness is essential in determining responses to intracellular and extracellular stresses. Although the nuclear membrane protein lamin A/C is known to contribute to nuclear stiffness, bulk moduli of nuclei have not been reported for various levels of lamin A/C. Here, we measure the nuclear bulk moduli as a function of lamin A/C expression and applied osmotic stress, revealing a linear dependence within the range of 2-4 MPa. We also find that the nuclear compression is anisotropic, with the vertical axis of the nucleus being more compliant than the minor and major axes in the substrate plane. We then rel

SUBMITTER: Srivastava LK 

PROVIDER: S-EPMC8186481 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature