RSC primes the quiescent genome for hypertranscription upon cell-cycle re-entry.

ABSTRACT: Quiescence is a reversible G₀ state essential for differentiation, regeneration, stem-cell renewal, and immune cell activation. Necessary for long-term survival, quiescent chromatin is compact, hypoacetylated, and transcriptionally inactive. How transcription activates upon cell-cycle re-entry is undefined. Here we report robust, widespread transcription within the first minutes of quiescence exit. During quiescence, the chromatin-remodeling enzyme RSC was already bound to the genes induced upon quiescence exit. RSC depletion caused severe quiescence exit defects: a global decrease in RNA polymerase II (Pol II) loading, Pol II accumulation at transcription start sites, initiation from ectopic upstream loci, and aberrant antisense transcription. These phenomena were due to a comb

SUBMITTER: Cucinotta CE 

PROVIDER: S-EPMC8186906 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature