Numerical tests of magnetoreception models assisted with behavioral experiments on American cockroaches.
Ontology highlight
ABSTRACT:
SUBMITTER: Lee KS
PROVIDER: S-EPMC8190300 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD(•-) and FADH(-) redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system.
| S-EPMC4760799 | biostudies-other
Project description:Behavioural tests of the magnetic compass of birds and corresponding immunohistological studies on the activation of retinal cryptochrome 1a, the putative receptor molecule, showed oriented behaviour and activated Cry1a under 373 nm UV, 424 nm blue, 502 nm turquoise and 565 nm green light, although the last wavelength does not allow the first step of photoreduction of cryptochrome to the semiquinone form. The tested birds had been kept under 'white' light before, hence we suggested that there was a supply of semiquinone present at the beginning of the exposure to green light that could be further reduced and then re-oxidized. To test the hypothesis in behavioural experiments, we tested robins, Erithacus rubecula, under various wavelengths (1) after 1 h pre-exposure to total darkness and (2) after 1 h pre-exposure to the same light as used in the test. The birds were oriented under blue and turquoise light, where the full cryptochrome cycle can run, but not under green light. This finding is in agreement with the hypothesis. Orientation under green light appears to be a transient phenomenon until the supply of semiquinone is depleted.
| S-EPMC4254397 | biostudies-other
Project description:It is becoming increasingly popular and straightforward to collect data in cognitive psychology through web-based studies. In this paper, I review issues around web-based data collection for the purpose of numerical cognition research. Provided that the desired type of data can be collected through a web-browser, such online studies offer numerous advantages over traditional forms of physical lab-based data collection, such as gathering data from larger sample sizes in shorter time-windows and easier access to non-local populations. I then present results of two replication studies that employ classical paradigms in numerical cognition research: the number-size congruity paradigm and comparison to a given standard, which also included a masked priming manipulation. In both replications, reaction times and error rates were comparable to original, physical lab-based studies. Consistent with the results of original studies, a distance effect, a congruity effect, and a priming effect were observed. Data collected online thus offers a level of reliability comparable to data collected in a physical lab when it comes to questions in numerical cognition.
| S-EPMC6753310 | biostudies-literature
Project description:The radical-pair hypothesis of magnetoreception has gained a lot of momentum, since the flavoprotein cryptochrome was postulated as a structural candidate to host magnetically sensitive chemical reactions. Here, we first discuss behavioral tests using radio-frequency magnetic fields (0.1-10 MHz) to specifically disturb a radical-pair-based avian magnetic compass sense. While disorienting effects of broadband RF magnetic fields have been replicated independently in two competing labs, the effects of monochromatic RF magnetic fields administered at the electronic Larmor frequency (~1.3 MHz) are disparate. We give technical recommendations for future RF experiments. We then focus on two candidate magnetoreceptor proteins in birds, Cry1a and Cry1b, two splice variants of the same gene (Cry1). Immunohistochemical studies have identified Cry1a in the outer segments of the ultraviolet/violet-sensitive cone photoreceptors and Cry1b in the cytosol of retinal ganglion cells. The identification of the host neurons of these cryptochromes and their subcellular expression patterns presents an important advance, but much work lies ahead to gain some functional understanding. In particular, interaction partners of cryptochrome Cry1a and Cry1b remain to be identified. A candidate partner for Cry4 was previously suggested, but awaits independent replication.
| S-EPMC5522499 | biostudies-other
Project description:Motor function in humans can be characterized with tests of locomotion, strength, balance, and endurance. The aim of our project was to establish an analogous test battery to assess motor function in mice. Male C57BL/6 mice were studied at 3 (n?=?87), 20 (n?=?48) and 26 (n?=?43) months of age. Tests assessed locomotion, strength, balance/coordination, and endurance capacity in mice. Motor function was reduced in the older groups of mice for the locomotion, strength, and endurance subdomains (p?<?0.001). As indicated with a summary score, motor function declined by 7.4 % from 3 to 20 months and by 13.5 % from 20 to 26 months. Based on comparison with previously published data in humans, the magnitude and relative time course of changes were similar in mice and humans in each subdomain except balance/coordination. Power calculations confirmed that the age-associated differences depicted by several of the individual tests and domain summary scores would be sufficient to assess the efficacy of interventions aimed at prevention or treatment of motor dysfunction with aging. The current study describes a mouse model that characterizes age-associated changes in clinically relevant domains of motor function and indicates that the preclinical model can be used to test strategies to attenuate age-associated declines in motor function.
| S-EPMC4039275 | biostudies-literature
Project description:Diverse animal species perceive Earth's magnetism and use their magnetic sense to orientate and navigate. Even non-migrating insects such as fruit flies and cockroaches have been shown to exploit the flavoprotein Cryptochrome (Cry) as a likely magnetic direction sensor; however, the transduction mechanism remains unknown. In order to work as a system to steer insect flight or control locomotion, the magnetic sense must transmit the signal from the receptor cells to the brain at a similar speed to other sensory systems, presumably within hundreds of milliseconds or less. So far, no electrophysiological or behavioral study has tackled the problem of the transduction delay in case of Cry-mediated magnetoreception specifically. Here, using a novel aversive conditioning assay on an American cockroach, we show that magnetic transduction is executed within a sub-second time span. A series of inter-stimulus intervals between conditioned stimuli (magnetic North rotation) and unconditioned aversive stimuli (hot air flow) provides original evidence that Cry-mediated magnetic transduction is sufficiently rapid to mediate insect orientation.
| S-EPMC5985609 | biostudies-literature
Project description:The administration of behavioral and experimental paradigms for psychology research is hindered by lack of a coordinated effort to develop and deploy standardized paradigms. While several frameworks (Mason and Suri, 2011; McDonnell et al., 2012; de Leeuw, 2015; Lange et al., 2015) have provided infrastructure and methods for individual research groups to develop paradigms, missing is a coordinated effort to develop paradigms linked with a system to easily deploy them. This disorganization leads to redundancy in development, divergent implementations of conceptually identical tasks, disorganized and error-prone code lacking documentation, and difficulty in replication. The ongoing reproducibility crisis in psychology and neuroscience research (Baker, 2015; Open Science Collaboration, 2015) highlights the urgency of this challenge: reproducible research in behavioral psychology is conditional on deployment of equivalent experiments. A large, accessible repository of experiments for researchers to develop collaboratively is most efficiently accomplished through an open source framework. Here we present the Experiment Factory, an open source framework for the development and deployment of web-based experiments. The modular infrastructure includes experiments, virtual machines for local or cloud deployment, and an application to drive these components and provide developers with functions and tools for further extension. We release this infrastructure with a deployment (http://www.expfactory.org) that researchers are currently using to run a set of over 80 standardized web-based experiments on Amazon Mechanical Turk. By providing open source tools for both deployment and development, this novel infrastructure holds promise to bring reproducibility to the administration of experiments, and accelerate scientific progress by providing a shared community resource of psychological paradigms.
| S-EPMC4844768 | biostudies-literature
Project description:Many distributed collective decision-making processes must balance diverse individual preferences with a desire for collective unity. We report here on an extensive session of behavioral experiments on biased voting in networks of individuals. In each of 81 experiments, 36 human subjects arranged in a virtual network were financially motivated to reach global consensus to one of two opposing choices. No payments were made unless the entire population reached a unanimous decision within 1 min, but different subjects were paid more for consensus to one choice or the other, and subjects could view only the current choices of their network neighbors, thus creating tensions between private incentives and preferences, global unity, and network structure. Along with analyses of how collective and individual performance vary with network structure and incentives generally, we find that there are well-studied network topologies in which the minority preference consistently wins globally; that the presence of "extremist" individuals, or the awareness of opposing incentives, reliably improve collective performance; and that certain behavioral characteristics of individual subjects, such as "stubbornness," are strongly correlated with earnings.
| S-EPMC2630202 | biostudies-literature
Project description:MotivationA gene set test is a differential expression analysis in which a P-value is assigned to a set of genes as a unit. Gene set tests are valuable for increasing statistical power, organizing and interpreting results and for relating expression patterns across different experiments. Existing methods are based on permutation. Methods that rely on permutation of probes unrealistically assume independence of genes, while those that rely on permutation of sample are suitable only for two-group comparisons with a good number of replicates in each group.ResultsWe present ROAST, a statistically rigorous gene set test that allows for gene-wise correlation while being applicable to almost any experimental design. Instead of permutation, ROAST uses rotation, a Monte Carlo technology for multivariate regression. Since the number of rotations does not depend on sample size, ROAST gives useful results even for experiments with minimal replication. ROAST allows for any experimental design that can be expressed as a linear model, and can also incorporate array weights and correlated samples. ROAST can be tuned for situations in which only a subset of the genes in the set are actively involved in the molecular pathway. ROAST can test for uni- or bi-direction regulation. Probes can also be weighted to allow for prior importance. The power and size of the ROAST procedure is demonstrated in a simulation study, and compared to that of a representative permutation method. Finally, ROAST is used to test the degree of transcriptional conservation between human and mouse mammary stems.AvailabilityROAST is implemented as a function in the Bioconductor package limma available from www.bioconductor.org.
| S-EPMC2922896 | biostudies-literature
Project description:The stress states in accretionary prisms are important for understanding the building and releasing of seismic energy. Numerous researchers have conducted sandbox experiments as a scaled physical analog model to understand the formation of accretionary prisms. However, measuring stress states in laboratory sandbox experiments is still practically infeasible. Here we performed real-scale numerical sandbox experiments using the discrete element method to understand the 3D stress state in the accretionary prism. Despite the nearly uniform initial conditions, macro-scale undulations of faults, which are similar to those observed in the trenches of an accretionary prism, appear. We reveal that these undulations are caused by the formation of stress arches. We show that the mechanism behind the arch formation is the discontinuous change in the stress orientation during the rearrangement of the stress chain. Furthermore, analyses demonstrate that the in-situ stress orientation from borehole data can be a signal of either the regional direction of plate convergence or the local stress orientation associated with the stress arch. The results may greatly enhance the outcome of long term monitoring in areas, such as the Nankai Trough.
| S-EPMC5993738 | biostudies-literature