Blocking siglec-10^hi tumor-associated macrophages improves anti-tumor immunity and enhances immunotherapy for hepatocellular carcinoma.

ABSTRACT:

Background

Tumor-associated macrophages (TAMs) promote key processes in the modulation of tumor microenvironment (TME). However, the clinical significance of heterogeneous subpopulations of TAMs in hepatocellular carcinoma (HCC) remains unknown.

Methods

HCC tissues from Zhongshan Hospital and data from The Cancer Genome Atlas were obtained and analyzed. Immunohistochemistry and flow cytometry were performed to detect the characteristics of sialic acid-binding immunoglobulin-like lectin 10^high (Siglec-10^hi) TAMs and explore their impact on the TME of HCC. The effect of Siglec-10 blockade was evaluated in vitro based on fresh human tumor tissues.

Results

Our data revealed that Siglec-10 was abundant in a large proportion of HCC specimens and promin

SUBMITTER: Xiao N 

PROVIDER: S-EPMC8191104 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature