Unknown

Dataset Information

0

Molecular Beacon DNA Probes with Fluorescein Bifluorophore.


ABSTRACT: An azido-derivative of a fluorescein bifluorophore was obtained and used for the synthesis of "molecular beacon"-type oligonucleotide fluorogenic probes for RT-PCR. Eight probe variants were synthesized based on an optimized sequence: with one or two quencher residues at the 3'-end, with a single or bifluorophore fluorescein label attached to 5'-end using modifying phosphoramidites (short linker) or "click reaction" (long linker). Comparison of probes in RT-PCR showed that probes with a doubled quencher (single fluorescein on a short linker) and doubled dye on a short linker (single dye) are somewhat superior in sensitivity to a standard probe (single quencher, single dye on a short linker) by the value of ΔCt = 1-2.

Supplementary information

The online version contains supplementary material available at 10.1134/S1068162021030055.

SUBMITTER: Brylev VA 

PROVIDER: S-EPMC8193957 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8156537 | biostudies-literature
| S-EPMC2668336 | biostudies-literature
| S-EPMC3000354 | biostudies-literature
| S-EPMC5433842 | biostudies-literature
| S-EPMC2025667 | biostudies-literature
| S-EPMC9187886 | biostudies-literature
| S-EPMC6114979 | biostudies-literature
| S-EPMC8235652 | biostudies-literature
| S-EPMC497581 | biostudies-other
| S-EPMC3000811 | biostudies-literature