In vivo Dominant-Negative Effect of an SCN5A Brugada Syndrome Variant.

ABSTRACT: Loss-of-function mutations in the cardiac Na⁺ channel α-subunit Na_v1.5, encoded by SCN5A, cause Brugada syndrome (BrS), a hereditary disease characterized by sudden cardiac death due to ventricular fibrillation. We previously evidenced in vitro the dominant-negative effect of the BrS Na_v1.5-R104W variant, inducing retention of wild-type (WT) channels and leading to a drastic reduction of the resulting Na⁺ current (I _Na ). To explore this dominant-negative effect in vivo, we created a murine model using adeno-associated viruses (AAVs).

Methods

Due to the large size of SCN5A, a dual AAV vector strategy was used combining viral DNA recombination and trans-splicing. Mice were injected with two AAV serotypes capsid 9: one packaging the cardiac specific troponin-T promoter, the 5' half of hSCN5A cDNA, a splicing donor site and a recombinogenic sequence; and another packaging the complementary recombinogenic sequence, a splicing acceptor site, the 3' half of hSCN5A cDNA fused to the gfp gene sequence, and the SV40 polyA signal. Eight weeks after AAV systemic injection in wild-type (WT) mice, echocardiography and ECG were recorded and mice were sacrificed. The full-length hSCN5A-gfp expression was assessed by western blot and immunohistochemistry in transduced heart tissues and the Na⁺ current was recorded by the patch-clamp technique in isolated adult GFP-expressing heart cells.

Results

Almost 75% of the cardiomyocytes were transduced in hearts of mice injected with hNa_v1.5 and ∼30% in hNa_v1.5-R104W overexpressing tissues. In ventricular mice cardiomyocytes expressing R104W mutant channels, the endogenous I _Na was significantly decreased. Moreover, overexpression of R104W channels in normal hearts led to a decrease of total Na_v1.5 expression. The R104W mutant also induced a slight dilatation of mice left ventricles and a prolongation of RR interval and P-wave duration in transduced mice. Altogether, our results demonstrated an in vivo dominant-negative effect of defective R104W channels on endogenous ones.

Conclusion

Using a trans-splicing and viral DNA recombination strategy to overexpress the Na⁺ channel in mouse hearts allowed us to demonstrate in vivo the dominant-negative effect of a BrS variant identified in the N-terminus of Na_v1.5.

SUBMITTER: Doisne N 

PROVIDER: S-EPMC8195286 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature